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CCR7 Modulates Pulmonary and Lymph Node Inflammatory Responses in Cigarette Smoke-Exposed Mice¹

Tine Demoor,^* Ken R. Bracke,^* Karim Y. Vermaelen, Lisa Dupont,^* Guy F. Joos,^* and Guy G. Brusselle^2*

*Department of Respiratory Medicine, Laboratory for Translational Research in Obstructive Pulmonary Diseases and Department of Respiratory Medicine, Laboratory of Immunoregulation and Mucosal Immunology, Ghent University Hospital, Ghent, Belgium

Peribronchial lymphoid follicles have recently been identified as one of the hallmark features of (severe) chronic obstructive pulmonary disease (COPD). However, little is known about the relative contribution of peribronchial lymphoid follicles vs mediastinal lymph nodes in inflammatory responses in COPD patients and animal models. In a murine model of COPD, we studied inflammatory responses in airways, lungs, and mediastinal lymph nodes of wild-type (WT) vs CCR7 knockout (CCR7^–/–) mice upon subacute or chronic exposure to cigarette smoke (CS). Although crucial for the organization of the secondary lymphoid organs, CCR7 was not required for the development of chronic CS-induced pulmonary lymphoid follicles. Moreover, T cell numbers were significantly increased in airways and lungs of air-exposed CCR7^–/– mice, and they continued to increase upon chronic CS exposure. Unexpectedly, subacute CS-induced inflammation in airways and lungs, including airway neutrophilia and the recruitment of inflammatory-type CD11b⁺ dendritic cells, depended greatly on CCR7. In the draining lymph nodes, chronic CS exposure induced CCR7-dependent recruitment of airway-derived dendritic cells, accompanied by increases in CD4⁺ and CD8⁺ T cells. Correspondingly, CS exposure up-regulated mRNA expression of CCR7 ligands CCL19 and CCL21-Ser in lymph nodes of WT mice, but not CCR7^–/– mice. In the lungs of WT mice, chronic CS exposure significantly increased CCL19 mRNA and protein. Furthermore, double staining for CCL19 and pro-surfactant protein C showed that alveolar type II cells express high levels of CCL19. These data unveil a so far unappreciated role for CCR7 in modulating inflammatory responses in airways and lungs.

² Address correspondence and reprint requests to Dr. Guy G. Brusselle, Department of Respiratory Medicine, Ghent University Hospital 7K12E, De Pintelaan 185, 9000 Ghent, Belgium. E-mail-address: guy.brusselle{at}ugent.be

¹ This work was supported by the Concerted Research Action of the University of Ghent (BOF/GOA 01251504; Ghent, Belgium), by the Fund for Scientific Research Flanders (Project G.0195.09N; Brussels, Belgium), and by the Interuniversity Attraction Poles Program/Belgian State/Belgian Science Policy (P6/35; Brussels, Belgium). K.B. is a Postdoctoral Fellow of the Fund for Scientific Research Flanders. K.V. is a Senior Clinical Investigator of the Fund for Scientific Research Flanders.

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				T. Demoor, K. R. Bracke, G. F. Joos, and G. G. Brusselle Increased T-regulatory cells in lungs and draining lymph nodes in a murine model of COPD Eur. Respir. J., March 1, 2010; 35(3): 688 - 689. [Full Text] [PDF]