The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fabry, Z.
Right arrow Articles by Sriram, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fabry, Z.
Right arrow Articles by Sriram, S.

The Journal of Immunology, Vol 155, Issue 1 325-332, Copyright © 1995 by American Association of Immunologists

ARTICLES

TGF-beta 2 decreases migration of lymphocytes in vitro and homing of cells into the central nervous system in vivo

Z Fabry, DJ Topham, D Fee, J Herlein, JA Carlino, MN Hart and S Sriram
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA.

Migration of leukocytes through an in vitro, cell culture model of the blood-brain barrier (BBB) composed of murine brain microvessel endothelial (En) cells and astrocytes, and in vivo in experimental allergic encephalomyelitis (EAE), was investigated. We have recently shown that the adhesiveness of cultured murine brain microvascular endothelial cells for lymphocytes can be increased significantly by pretreatment with IL-1 beta, TNF-alpha, IFN-gamma, and LPS. In the present study, we investigated the role of TGF-beta 2 on the migration of leukocytes through the BBB. In vitro migration was assessed by measuring the percentage of 51Cr-labeled leukocytes migrating through the En/astrocyte monolayers. The basal level of migration was up- regulated significantly by treating the En/astrocyte monolayers with IL- 1 alpha, IFN-gamma, TNF-alpha, and LPS. The ability of these cytokines to modulate migration was dose-dependent. Treatment of En cell/astrocyte monolayers with TGF-beta 2 down-regulated the level of leukocyte migration up-regulated by IL-1 alpha, IFN-gamma, and TNF- alpha in vitro in a dose-dependent manner. TGF-beta 2 also inhibited the migration of lymphocytes into the central nervous system (CNS) in vivo in a dose-dependent fashion. Taken together, these findings strongly suggest that TGF-beta plays an important role in the reduction of lymphocyte infiltration into the CNS in inflammatory demyelinating diseases such as EAE.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y. Sonobe, H. Takeuchi, K. Kataoka, H. Li, S. Jin, M. Mimuro, Y. Hashizume, Y. Sano, T. Kanda, T. Mizuno, et al.
Interleukin-25 Expressed by Brain Capillary Endothelial Cells Maintains Blood-Brain Barrier Function in a Protein Kinase C{epsilon}-dependent Manner
J. Biol. Chem., November 13, 2009; 284(46): 31834 - 31842.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M.-L. Cheng, H.-W. Chen, J.-P. Tsai, Y.-P. Lee, Y.-C. Shih, C.-M. Chang, and C.-C. Ting
Clonal restriction of the expansion of antigen-specific CD8+ memory T cells by transforming growth factor-{beta}
J. Leukoc. Biol., May 1, 2006; 79(5): 1033 - 1042.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Murano, X. Xiong, N. Murano, J. L. Salzer, J. J. Lafaille, and V. K. Tsiagbe
Latent TGF-{beta}1-transduced CD4+ T cells suppress the progression of allergic encephalomyelitis
J. Leukoc. Biol., January 1, 2006; 79(1): 140 - 146.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Subramanian, A. Matejuk, A. Zamora, A. A. Vandenbark, and H. Offner
Oral Feeding with Ethinyl Estradiol Suppresses and Treats Experimental Autoimmune Encephalomyelitis in SJL Mice and Inhibits the Recruitment of Inflammatory Cells into the Central Nervous System
J. Immunol., February 1, 2003; 170(3): 1548 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
A. Faber-Elmann, V. Grabovsky, M. Dayan, M. Sela, R. Alon, and E. Mozes
Cytokine profile and T cell adhesiveness to endothelial selectins: in vivo induction by a myasthenogenic T cell epitope and immunomodulation by a dual altered peptide ligand
Int. Immunol., December 1, 2000; 12(12): 1651 - 1658.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F.M. Hofman, P. Chen, R. Jeyaseelan, F. Incardona, M. Fisher, and R. Zidovetzki
Endothelin-1 Induces Production of the Neutrophil Chemotactic Factor Interleukin-8 by Human Brain-Derived Endothelial Cells
Blood, November 1, 1998; 92(9): 3064 - 3072.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Z. Chen, G. M. Hochwald, C. Huang, G. Dakin, H. Tao, C. Cheng, W. J. Simmons, G. Dranoff, and G. J. Thorbecke
Gene therapy in allergic encephalomyelitis using myelin basic protein-specific T cells engineered to express latent transforming growth factor-beta 1
PNAS, October 13, 1998; 95(21): 12516 - 12521.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Trocme, P. Gaudin, S. Berthier, C. Barro, P. Zaoui, and F. Morel
Human B Lymphocytes Synthesize the 92-kDa Gelatinase, Matrix Metalloproteinase-9
J. Biol. Chem., August 7, 1998; 273(32): 20677 - 20684.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. J. D'Orazio and J. Y. Niederkorn
A Novel Role for TGF-{beta} and IL-10 in the Induction of Immune Privilege
J. Immunol., March 1, 1998; 160(5): 2089 - 2098.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.