The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lebel-Binay, S.
Right arrow Articles by Conjeaud, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lebel-Binay, S.
Right arrow Articles by Conjeaud, H.

The Journal of Immunology, Vol 155, Issue 1 101-110, Copyright © 1995 by American Association of Immunologists

ARTICLES

CD82, member of the tetra-span-transmembrane protein family, is a costimulatory protein for T cell activation

S Lebel-Binay, C Lagaudriere, D Fradelizi and H Conjeaud
Immunomodulation and Autoimmunity Laboratory, Rene Descartes University, Cochin Hospital, Paris, France.

It is now well documented that full activation of T cells requires a two-signal triggering that can be mimicked, in the absence of accessory cells, by co-immobilization of mAbs directed to stimulatory/accessory molecules (CD2, CD3, CD28, adhesion molecules, etc.). In this report, we describe that engagement of CD82 can delivery such a costimulatory signal for full activation of the human T cell line Jurkat, leading to strong IL-2 production and cell differentiation. The CD82 Ag, which belongs to the new tetra-span-transmembrane family (CD9, CD37, CD53, CD63, and CD81 (TAPA-1)), has been identified originally in our laboratory for its enhanced expression on three LAK-susceptible cell lines, and has been characterized as an activation/differentiation marker of mononuclear cells. Jurkat cells, stimulated in vitro by co- immobilization of anti-CD82 and anti-CD3 mAbs, produced high levels of IL-2, became strongly adherent to plastic dishes, and developed dendritic processes. These morphologic changes, associated with a total arrest of cell proliferation, were not the result of cell death but rather of cell differentiation, as shown by an increase in their metabolic activity. Costimulation through both CD82 and CD3 induced up- regulation of both IL-2 and IFN-gamma mRNA synthesis (but not of IL-4) and an increased expression of HLA class I molecules at the cell surface, which was inhibited by anti-IFN-gamma Ab.


This article has been cited by other articles:


Home page
 Cancer Res.Home page
M. Iiizumi, S. Bandyopadhyay, and K. Watabe
Interaction of Duffy Antigen Receptor for Chemokines and KAI1: A Critical Step in Metastasis Suppression
Cancer Res., February 15, 2007; 67(4): 1411 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Pfistershammer, O. Majdic, J. Stockl, G. Zlabinger, S. Kirchberger, P. Steinberger, and W. Knapp
CD63 as an Activation-Linked T Cell Costimulatory Element
J. Immunol., November 15, 2004; 173(10): 6000 - 6008.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Delaguillaumie, J. Harriague, S. Kohanna, G. Bismuth, E. Rubinstein, M. Seigneuret, and H. Conjeaud
Tetraspanin CD82 controls the association of cholesterol-dependent microdomains with the actin cytoskeleton in T lymphocytes: relevance to co-stimulation
J. Cell Sci., October 15, 2004; 117(22): 5269 - 5282.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. McCarty, M. L. Shinohara, L. Lu, and H. Cantor
Detailed analysis of gene expression during development of T cell lineages in the thymus
PNAS, June 22, 2004; 101(25): 9339 - 9344.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Imamura, T. Katahira, and D. Kitamura
Identification and Characterization of a Novel BASH N Terminus-associated Protein, BNAS2
J. Biol. Chem., June 18, 2004; 279(25): 26425 - 26432.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. B. van Spriel, K. L. Puls, M. Sofi, D. Pouniotis, H. Hochrein, Z. Orinska, K.-P. Knobeloch, M. Plebanski, and M. D. Wright
A Regulatory Role for CD37 in T Cell Proliferation
J. Immunol., March 1, 2004; 172(5): 2953 - 2961.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. H. Lee, Y.-W. Seo, S. R. Park, Y. J. Kim, and K. K. Kim
Expression of a Splice Variant of KAI1, a Tumor Metastasis Suppressor Gene, Influences Tumor Invasion and Progression
Cancer Res., November 1, 2003; 63(21): 7247 - 7255.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. A. Zhang, B. He, B. Zhou, and L. Liu
Requirement of the p130CAS-Crk Coupling for Metastasis Suppressor KAI1/CD82-mediated Inhibition of Cell Migration
J. Biol. Chem., July 11, 2003; 278(29): 27319 - 27328.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. A. Zhang, W. S. Lane, S. Charrin, E. Rubinstein, and L. Liu
EWI2/PGRL Associates with the Metastasis Suppressor KAI1/CD82 and Inhibits the Migration of Prostate Cancer Cells
Cancer Res., May 15, 2003; 63(10): 2665 - 2674.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. M. Tarrant, J. Groom, D. Metcalf, R. Li, B. Borobokas, M. D. Wright, D. Tarlinton, and L. Robb
The Absence of Tssc6, a Member of the Tetraspanin Superfamily, Does Not Affect Lymphoid Development but Enhances In Vitro T-Cell Proliferative Responses
Mol. Cell. Biol., July 15, 2002; 22(14): 5006 - 5018.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Delaguillaumie, C. Lagaudriere-Gesbert, M. R. Popoff, and H. Conjeaud
Rho GTPases link cytoskeletal rearrangements and activation processes induced via the tetraspanin CD82 in T lymphocytes
J. Cell Sci., January 15, 2002; 115(2): 433 - 443.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. A. Witherden, R. Boismenu, and W. L. Havran
CD81 and CD28 Costimulate T Cells Through Distinct Pathways
J. Immunol., August 15, 2000; 165(4): 1902 - 1909.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
K. Toyo-oka, Y. Yashiro-Ohtani, C.-S. Park, X.-G. Tai, K. Miyake, T. Hamaoka, and H. Fujiwara
Association of a tetraspanin CD9 with CD5 on the T cell surface: role of particular transmembrane domains in the association
Int. Immunol., December 1, 1999; 11(12): 2043 - 2052.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. E. Testa, P. C. Brooks, J.-M. Lin, and J. P. Quigley
Eukaryotic Expression Cloning with an Antimetastatic Monoclonal Antibody Identifies a Tetraspanin (PETA-3/CD151) as an Effector of Human TumorCell Migration and Metastasis
Cancer Res., August 1, 1999; 59(15): 3812 - 3820.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-M. Escola, M. J. Kleijmeer, W. Stoorvogel, J. M. Griffith, O. Yoshie, and H. J. Geuze
Selective Enrichment of Tetraspan Proteins on the Internal Vesicles of Multivesicular Endosomes and on Exosomes Secreted by Human B-lymphocytes
J. Biol. Chem., August 7, 1998; 273(32): 20121 - 20127.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Yanez-Mo, A. Alfranca, C. Cabanas, M. Marazuela, R. Tejedor, M. Angeles Ursa, L. K. Ashman, M. O. de Landazuri, and F. Sanchez-Madrid
Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with {alpha}3{beta}1 Integrin Localized at Endothelial Lateral Junctions
J. Cell Biol., May 4, 1998; 141(3): 791 - 804.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.