The Journal of Immunology, Vol 149, Issue 1 53-59, Copyright © 1992 by American Association of Immunologists

ARTICLES

Induction of a cellular immune response to a foreign antigen by a recombinant Listeria monocytogenes vaccine

R Schafer, DA Portnoy, SA Brassell and Y Paterson
Department of Microbiology, University of Pennsylvania, Philadelphia 19104.

A recombinant strain of Listeria monocytogenes that stably and constitutively expresses Escherichia coli beta-galactosidase was used as a live vaccine vector. BALB/c mice were immunized orally or parenterally with the recombinant L. monocytogenes, and their cellular and humoral immune responses to beta-galactosidase were measured. Spleen cells taken 1 week after oral inoculation or 5 weeks after oral or parenteral inoculation (with a boost at 4 weeks) showed beta- galactosidase-specific CTL responses. The CTL line derived from mice immunized i.p. was also shown to be class I restricted and Thy-1.2+, CD8+, and TCR alpha beta+. All mice immunized with the recombinant L. monocytogenes had positive delayed-type hypersensitivity responses to heat-killed L. monocytogenes, but only 15% had a positive delayed-type hypersensitivity reaction to beta-galactosidase. Individual serum samples from mice immunized i.p. or i.v. were tested for antibody to beta-galactosidase. Approximately 11% had low positive titers for beta- galactosidase antibodies. These results demonstrate that both oral and parenteral immunization with recombinant L. monocytogenes results in a cellular immune response to the foreign protein, which is primarily a specific CD8+ CTL response.

This article has been cited by other articles:

				P. C. Maciag, M. M. Seavey, Z.-K. Pan, S. Ferrone, and Y. Paterson Cancer Immunotherapy Targeting the High Molecular Weight Melanoma-Associated Antigen Protein Results in a Broad Antitumor Response and Reduction of Pericytes in the Tumor Vasculature Cancer Res., October 1, 2008; 68(19): 8066 - 8075. [Abstract] [Full Text] [PDF]

				Z. Li, X. Zhao, C. Zhou, B. Gu, and F. R. Frankel A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Microbiology, October 1, 2006; 152(Pt 10): 3091 - 3102. [Abstract] [Full Text] [PDF]

				Z. Li, X. Zhao, D. E. Higgins, and F. R. Frankel Conditional Lethality Yields a New Vaccine Strain of Listeria monocytogenes for the Induction of Cell-Mediated Immunity Infect. Immun., August 1, 2005; 73(8): 5065 - 5073. [Abstract] [Full Text] [PDF]

				N. Walters, T. Trunkle, M. Sura, and D. W. Pascual Enhanced Immunoglobulin A Response and Protection against Salmonella enterica Serovar Typhimurium in the Absence of the Substance P Receptor Infect. Immun., January 1, 2005; 73(1): 317 - 324. [Abstract] [Full Text] [PDF]

				C. N. Kotton and E. L. Hohmann Enteric Pathogens as Vaccine Vectors for Foreign Antigen Delivery Infect. Immun., October 1, 2004; 72(10): 5535 - 5547. [Full Text] [PDF]

				C. Peters, X. Peng, D. Douven, Z.-K. Pan, and Y. Paterson The Induction of HIV Gag-Specific CD8+ T Cells in the Spleen and Gut-Associated Lymphoid Tissue by Parenteral or Mucosal Immunization with Recombinant Listeria monocytogenes HIV Gag J. Immunol., May 15, 2003; 170(10): 5176 - 5187. [Abstract] [Full Text] [PDF]

				M. S. Di Genaro, M. Waidmann, U. Kramer, N. Hitziger, E. Bohn, and I. B. Autenrieth Attenuated Yersiniaenterocolitica Mutant Strains Exhibit Differential Virulence in Cytokine-Deficient Mice: Implications for the Development of Novel Live Carrier Vaccines Infect. Immun., April 1, 2003; 71(4): 1804 - 1812. [Abstract] [Full Text]

				H. Angelakopoulos, K. Loock, D. M. Sisul, E. R. Jensen, J. F. Miller, and E. L. Hohmann Safety and Shedding of an Attenuated Strain of Listeria monocytogenes with a Deletion of actA/plcB in Adult Volunteers: a Dose Escalation Study of Oral Inoculation Infect. Immun., July 1, 2002; 70(7): 3592 - 3601. [Abstract] [Full Text] [PDF]

				L. M. Liau, E. R. Jensen, T. J. Kremen, S. K. Odesa, S. N. Sykes, M. C. Soung, J. F. Miller, and J. M. Bronstein Tumor Immunity within the Central Nervous System Stimulated by Recombinant Listeria monocytogenes Vaccination Cancer Res., April 1, 2002; 62(8): 2287 - 2293. [Abstract] [Full Text] [PDF]

				A. R. Tvinnereim, S. E. Hamilton, and J. T. Harty CD8+-T-Cell Response to Secreted and Nonsecreted Antigens Delivered by Recombinant Listeria monocytogenes during Secondary Infection Infect. Immun., January 1, 2002; 70(1): 153 - 162. [Abstract] [Full Text] [PDF]

				D. W. Pascual, M. D. White, T. Larson, and N. Walters Impaired Mucosal Immunity in L-Selectin-Deficient Mice Orally Immunized with a Salmonella Vaccine Vector J. Immunol., July 1, 2001; 167(1): 407 - 415. [Abstract] [Full Text] [PDF]

				M. Mata and Y. Paterson Th1 T Cell Responses to HIV-1 Gag Protein Delivered by a Listeria monocytogenes Vaccine Are Similar to Those Induced by Endogenous Listerial Antigens J. Immunol., August 1, 1999; 163(3): 1449 - 1456. [Abstract] [Full Text] [PDF]

				T.R.J. Evans and S.B. Kaye Vaccine therapy for cancer—fact or fiction? QJM, June 1, 1999; 92(6): 299 - 307. [Full Text] [PDF]

				S. Vijh, I. M. Pilip, and E. G. Pamer Noncompetitive Expansion of Cytotoxic T Lymphocytes Specific for Different Antigens during Bacterial Infection Infect. Immun., March 1, 1999; 67(3): 1303 - 1309. [Abstract] [Full Text] [PDF]

				H. G. A. Bouwer, H. Shen, X. Fan, J. F. Miller, R. A. Barry, and D. J. Hinrichs Existing Antilisterial Immunity Does Not Inhibit the Development of a Listeria monocytogenes-Specific Primary Cytotoxic T-Lymphocyte Response Infect. Immun., January 1, 1999; 67(1): 253 - 258. [Abstract] [Full Text] [PDF]

				M. Mata, P. J. Travers, Q. Liu, F. R. Frankel, and Y. Paterson The MHC Class I-Restricted Immune Response to HIV-gag in BALB/c Mice Selects a Single Epitope That Does Not Have a Predictable MHC-Binding Motif and Binds to Kd Through Interactions Between a Glutamine at P3 and Pocket D J. Immunol., September 15, 1998; 161(6): 2985 - 2993. [Abstract] [Full Text] [PDF]

				J. Ulmer, J. Donnelly, S. Parker, G. Rhodes, P. Felgner, V. Dwarki, S. Gromkowski, R. Deck, C. DeWitt, A Friedman, et al. Heterologous protection against influenza by injection of DNA encoding a viral protein Science, March 19, 1993; 259(5102): 1745 - 1749. [Abstract] [PDF]