The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mathison, J. C.
Right arrow Articles by Ulevitch, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mathison, J. C.
Right arrow Articles by Ulevitch, R. J.

The Journal of Immunology, Vol 149, Issue 1 200-206, Copyright © 1992 by American Association of Immunologists

ARTICLES

Plasma lipopolysaccharide (LPS)-binding protein. A key component in macrophage recognition of gram-negative LPS

JC Mathison, PS Tobias, E Wolfson and RJ Ulevitch
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.

LPS-binding protein (LBP) binds with high affinity (Kd approximately equal to 10(-9) M) to lipid A of LPS isolated from rough (R)- or smooth (S)-form Gram-negative bacteria as well as to lipid A partial structures such as precursor IVA. To define the role of LBP in regulating responses to LPS we have examined TNF release in rabbit peritoneal exudate macrophages (M phi) stimulated with LPS or with complete or partial lipid A preparations in the presence or absence of LBP. In the presence of LBP, M phi showed increased sensitivity to S- and R-form LPS as well as synthetic lipid A. Compared with LPS or lipid A, up to 1000-fold greater concentrations of partial lipid A structures were required to induce TNF production. However, consistent with our previous observations that these structures bind to LBP, TNF production was increased in the presence of LBP. In contrast, LBP did not enhance or inhibit TNF production produced by heat-killed Staphylococcus aureus, peptidoglycan isolated from S. aureus cell walls, or PMA. Potentiated M phi responsiveness to LPS was observed with as little as 1 ng LBP/ml. Heat-denatured LBP (which no longer binds LPS), BPI (an homologous LPS-binding protein isolated from neutrophils), or other serum proteins were without effect. LBP-treated M phi also showed a more rapid induction of cytokine mRNA (TNF and IL-1 beta), higher steady-state mRNA levels and increased TNF mRNA stability. These data provide additional evidence that LBP is part of a highly specific recognition system controlling M phi responses to LPS. The effects of LBP are lipid A dependent and importantly, extend to LPS preparations isolated from bacteria of R- and S-form phenotype.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. H. Youn, Y. J. Oh, E. S. Kim, J. E. Choi, and J.-S. Shin
High Mobility Group Box 1 Protein Binding to Lipopolysaccharide Facilitates Transfer of Lipopolysaccharide to CD14 and Enhances Lipopolysaccharide-Mediated TNF-{alpha} Production in Human Monocytes
J. Immunol., April 1, 2008; 180(7): 5067 - 5074.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Rouhiainen, S. Tumova, L. Valmu, N. Kalkkinen, and H. Rauvala
Analysis of proinflammatory activity of highly purified eukaryotic recombinant HMGB1 (amphoterin)
J. Leukoc. Biol., January 1, 2007; 81(1): 49 - 58.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Lehnert, T. Uehara, B. U. Bradford, H. Lind, Z. Zhong, D. A. Brenner, I. Marzi, and J. J. Lemasters
Lipopolysaccharide-binding protein modulates hepatic damage and the inflammatory response after hemorrhagic shock and resuscitation
Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G456 - G463.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
N. W.J. Schroder and R. R. Schumann
Non-LPS targets and actions of LPS binding protein (LBP)
Innate Immunity, August 1, 2005; 11(4): 237 - 242.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
C. Habich, K. Kempe, R. van der Zee, R. Rumenapf, H. Akiyama, H. Kolb, and V. Burkart
Heat Shock Protein 60: Specific Binding of Lipopolysaccharide
J. Immunol., February 1, 2005; 174(3): 1298 - 1305.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
J. Branger, S. Florquin, S. Knapp, J. C. Leemans, J. M. Pater, P. Speelman, D. T. Golenbock, and T. van der Poll
LPS-binding protein-deficient mice have an impaired defense against Gram-negative but not Gram-positive pneumonia
Int. Immunol., November 1, 2004; 16(11): 1605 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
M. d. J. Arana, M. G. Vallespi, G. Chinea, G. V. Vallespi, I. Rodriguez-Alonso, H. E. Garay, W. A. Buurman, and O. Reyes
Inhibition of LPS-responses by synthetic peptides derived from LBP associates with the ability of the peptides to block LBP-LPS interaction
Innate Immunity, October 1, 2003; 9(5): 281 - 291.
[Abstract] [PDF]

Home page
 J DAIRY SCIHome page
D. D. Bannerman, M. J. Paape, W. R. Hare, and E. J. Sohn
Increased Levels of LPS-Binding Protein in Bovine Blood and Milk Following Bacterial Lipopolysaccharide Challenge
J Dairy Sci, October 1, 2003; 86(10): 3128 - 3137.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
E. S. Van Amersfoort, T. J. C. Van Berkel, and J. Kuiper
Receptors, Mediators, and Mechanisms Involved in Bacterial Sepsis and Septic Shock
Clin. Microbiol. Rev., July 1, 2003; 16(3): 379 - 414.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. A. Majcherczyk, E. Rubli, D. Heumann, M. P. Glauser, and P. Moreillon
Teichoic Acids Are Not Required for Streptococcus pneumoniae and Staphylococcus aureus Cell Walls To Trigger the Release of Tumor Necrosis Factor by Peripheral Blood Monocytes
Infect. Immun., July 1, 2003; 71(7): 3707 - 3713.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
A J Kvalsvig and D J Unsworth
The immunopathogenesis of meningococcal disease
J. Clin. Pathol., June 1, 2003; 56(6): 417 - 422.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Uesugi, M. Froh, G. E. Arteel, B. U. Bradford, M. D. Wheeler, E. Gabele, F. Isayama, and R. G. Thurman
Role of Lipopolysaccharide-Binding Protein in Early Alcohol-Induced Liver Injury in Mice
J. Immunol., March 15, 2002; 168(6): 2963 - 2969.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. Vassallo, T. J. Kottom, J. E. Standing, and A. H. Limper
Vitronectin and Fibronectin Function as Glucan Binding Proteins Augmenting Macrophage Responses to Pneumocystis carinii
Am. J. Respir. Cell Mol. Biol., August 1, 2001; 25(2): 203 - 211.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Cuzzola, G. Mancuso, C. Beninati, C. Biondo, F. Genovese, F. Tomasello, T. H. Flo, T. Espevik, and G. Teti
{beta}2 Integrins Are Involved in Cytokine Responses to Whole Gram-Positive Bacteria
J. Immunol., June 1, 2000; 164(11): 5871 - 5876.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
A N Kimmings, S J H van Deventer, H Obertop, E A J Rauws, K Huibregtse, and D J Gouma
Endotoxin, cytokines, and endotoxin binding proteins in obstructive jaundice and after preoperative biliary drainage
Gut, May 1, 2000; 46(5): 725 - 731.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Cuzzola, G. Mancuso, C. Beninati, C. Biondo, C. von Hunolstein, G. Orefici, T. Espevik, T. H. Flo, and G. Teti
Human Monocyte Receptors Involved in Tumor Necrosis Factor Responses to Group B Streptococcal Products
Infect. Immun., February 1, 2000; 68(2): 994 - 998.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. R. Ingalls, B. G. Monks, and D. T. Golenbock
Membrane Expression of Soluble Endotoxin-binding Proteins Permits Lipopolysaccharide Signaling in Chinese Hamster Ovary Fibroblasts Independently of CD14
J. Biol. Chem., May 14, 1999; 274(20): 13993 - 13998.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. Grunfeld, M. Marshall, J. K. Shigenaga, A. H. Moser, P. Tobias, and K. R. Feingold
Lipoproteins inhibit macrophage activation by lipoteichoic acid
J. Lipid Res., February 1, 1999; 40(2): 245 - 252.
[Abstract] [Full Text]

Home page
 Innate ImmunityHome page
R. Dziarski and D. Gupta
Function of CD14 as a peptidoglycan receptor: differences and similarities with LPS
Innate Immunity, February 1, 1999; 5(1-2): 56 - 61.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
R. R. Ingalls, B. G. Monks, R. Savedra Jr., W. J. Christ, R. L. Delude, A. E. Medvedev, T. Espevik, and D. T. Golenbock
CD11/CD18 and CD14 Share a Common Lipid A Signaling Pathway
J. Immunol., November 15, 1998; 161(10): 5413 - 5420.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. M. Wurfel, B. G. Monks, R. R. Ingalls, R. L. Dedrick, R. Delude, D. Zhou, N. Lamping, R. R. Schumann, R. Thieringer, M. J. Fenton, et al.
Targeted Deletion of the Lipopolysaccharide (LPS)-binding Protein Gene Leads to Profound Suppression of LPS Responses Ex Vivo, whereas In Vivo Responses Remain Intact
J. Exp. Med., December 15, 1997; 186(12): 2051 - 2056.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
N. Hirohashi, M. Richards, and D.C. Morrison
Selective effects of serum on bacterial LPS-induced IL-6 and nitric oxide production in murine peritoneal macrophages
Innate Immunity, October 1, 1996; 3(5): 395 - 405.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
D. Gupta, T. N. Kirkland, S. Viriyakosol, and R. Dziarski
CD14 Is a Cell-activating Receptor for Bacterial Peptidoglycan
J. Biol. Chem., September 20, 1996; 271(38): 23310 - 23316.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
M.C. Falk, M.A. Fletcher, T.J. Williams, W.M. Ching, Y. Wu, and T.K. Morrison
Aggregation of serum proteins with lipopolysaccharide (LPS): characterization of the precipitable LPS-protein complex
Innate Immunity, April 1, 1996; 3(2): 129 - 142.
[Abstract] [PDF]

Home page
 Arch SurgHome page
R. J. Burnett, C. A. Lyden, C. J. Tindal, C. M. Cave, M. N. Marra, and J. S. Solomkin
Mononuclear Cell Line THP-1 Internalizes Bactericidal/Permeability-Increasing Protein by a Non--Receptor-Mediated Mechanism Consistent With Pinocytosis
Arch Surg, February 1, 1996; 131(2): 200 - 206.
[Abstract] [PDF]

Home page
 Innate ImmunityHome page
R.J. Burnett, C.A. Lyden, C.J. Tindal, M.N. Marra, A.N. Neely, and J.S. Solomkin
Effects of bactericidal/permeability-increasing protein upon mononuclear cell interactions with endotoxin: evidence for cell binding
Innate Immunity, October 1, 1995; 2(5): 349 - 357.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. H. Taylor, G. Heavner, M. Nedelman, D. Sherris, E. Brunt, D. Knight, and J. Ghrayeb
Lipopolysaccharide (LPS) Neutralizing Peptides Reveal a Lipid A Binding Site of LPS Binding Protein
J. Biol. Chem., July 28, 1995; 270(30): 17934 - 17938.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. S. Tobias, K. Soldau, J. A. Gegner, D. Mintz, and R. J. Ulevitch
Lipopolysaccharide Binding Protein-mediated Complexation of Lipopolysaccharide with Soluble CD14
J. Biol. Chem., May 5, 1995; 270(18): 10482 - 10488.
[Abstract] [Full Text] [PDF]

Home page
 Arch SurgHome page
S. E. Calvano, W. A. Thompson, M. N. Marra, S. M. Coyle, H. F. de Riesthal, R. K. Trousdale, P. S. Barie, R. W. Scott, L. L. Moldawer, and S. F. Lowry
Changes in Polymorphonuclear Leukocyte Surface and Plasma Bactericidal/Permeability-Increasing Protein and Plasma Lipopolysaccharide Binding Protein During Endotoxemia or Sepsis
Arch Surg, February 1, 1994; 129(2): 220 - 226.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1992 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1992 by The American Association of Immunologists, Inc. All rights reserved.